The integrated forms of the S1 and S2 DNA elements of maize male sterile mitochondrial DNA are flanked by a large repeated sequence

The mitochondrial DNA of maize was cloned using the cosmid, Homer I. Recombinants carrying sequences homologous to the S1 and S2 DNA elements of male sterile maize have been analysed. Restriction endonuclease maps for Sac II, Sma I and Bam HI have been constructed.The S1 and S2 sequences are single...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1981-08, Vol.9 (15), p.3657-3669
Main Authors: Lonsdale, D.M., Thompson, R.D., Hodge, T.P.
Format: Article
Language:English
Subjects:
Base Sequence
Cloning, Molecular
DNA Replication
DNA Restriction Enzymes
DNA, Mitochondrial - genetics
DNA, Recombinant - metabolism
Genetic Vectors
Plants - genetics
Plasmids
Zea mays
Zea mays - genetics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The mitochondrial DNA of maize was cloned using the cosmid, Homer I. Recombinants carrying sequences homologous to the S1 and S2 DNA elements of male sterile maize have been analysed. Restriction endonuclease maps for Sac II, Sma I and Bam HI have been constructed.The S1 and S2 sequences are single copy sequences occurring at unique sites; each is flanked by a 26 kb repeated sequence. The repeated sequence hasbeen shown not to contain the mitochondrial ribosomal RNA genes.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/9.15.3657