Transcription of the β-like globin genes and pseudogenes of the goat in a cell-free system

We have examined the transcription in a cell-free system of all the members of a highly regulated gene family, the β-like hemoglobin genes of the goat. These five genes, which code for embryonic, fetal, juvenile, and adult β-globin proteins, are all transcribed to roughly the same extent in the in v...

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Bibliographic Details
Published in:Nucleic acids research 1981-09, Vol.9 (17), p.4339-4354
Main Authors: Luse, D.S., Haynes, J.R., VanLeeuwen, D., Schon, E.A., Cleary, M.L., Shapiro, S.G., Lingrel, J.B., Roeder, R.G.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell-Free System
DNA - analysis
DNA Restriction Enzymes - metabolism
Electrophoresis, Polyacrylamide Gel
Globins - genetics
Goats - genetics
Mutation
Transcription, Genetic
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Summary:We have examined the transcription in a cell-free system of all the members of a highly regulated gene family, the β-like hemoglobin genes of the goat. These five genes, which code for embryonic, fetal, juvenile, and adult β-globin proteins, are all transcribed to roughly the same extent in the in vitro system. In all cases initiation of transcription is accurate. However, two goat β-globin pseudogenes, as well as several artificially constructed deletion mutants, are not transcribed in vitro. A common feature of the transcriptionally inactive genes is the lack of an AT-rich consensus sequence just upstream of the presumptive initiation site.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/9.17.4339