Adenovirus late sequences linked to herpes simplex virus thymidine kinase may be introduced into eukaryotic cells and transcribed

LTK− cells have been transformed to the TK+ phenotype by treatment with size-defined concatamers of HSV-1 TK DNA and Ad2 Bam HI C fragment (42.0 − 59.5 map units). All TK+ transformants contained Ad2 DNA as well as HSV-1 TK sequences. In most cases several inserts of virus DNA were present, many in...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1981-11, Vol.9 (21), p.5569-5585
Main Authors: Inglis, Moira M., Darby, Graham
Format: Article
Language:English
Subjects:
Adenovirus
Adenoviruses, Human - enzymology
Adenoviruses, Human - genetics
Animals
Base Sequence
Cell Transformation, Viral
DNA, Viral - genetics
Herpes simplex virus 1
L Cells (Cell Line) - enzymology
Mice
Nucleic Acid Hybridization
Simplexvirus - enzymology
Simplexvirus - genetics
Thymidine Kinase - genetics
Transcription, Genetic
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:LTK− cells have been transformed to the TK+ phenotype by treatment with size-defined concatamers of HSV-1 TK DNA and Ad2 Bam HI C fragment (42.0 − 59.5 map units). All TK+ transformants contained Ad2 DNA as well as HSV-1 TK sequences. In most cases several inserts of virus DNA were present, many in high copy numbers. Although no Ad2 transcription promoter was present in the transforming DNA, Ad2-specific sequences were detected in polyadenylated cytoplasmic RNA species from several cell lines.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/9.21.5569