Adenovirus late sequences linked to herpes simplex virus thymidine kinase may be introduced into eukaryotic cells and transcribed

LTK− cells have been transformed to the TK+ phenotype by treatment with size-defined concatamers of HSV-1 TK DNA and Ad2 Bam HI C fragment (42.0 − 59.5 map units). All TK+ transformants contained Ad2 DNA as well as HSV-1 TK sequences. In most cases several inserts of virus DNA were present, many in...

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Published in:Nucleic acids research 1981-11, Vol.9 (21), p.5569-5585
Main Authors: Inglis, Moira M., Darby, Graham
Format: Article
Language:English
Subjects:
Adenovirus
Adenoviruses, Human - enzymology
Adenoviruses, Human - genetics
Animals
Base Sequence
Cell Transformation, Viral
DNA, Viral - genetics
Herpes simplex virus 1
L Cells (Cell Line) - enzymology
Mice
Nucleic Acid Hybridization
Simplexvirus - enzymology
Simplexvirus - genetics
Thymidine Kinase - genetics
Transcription, Genetic
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Darby, Graham
description LTK− cells have been transformed to the TK+ phenotype by treatment with size-defined concatamers of HSV-1 TK DNA and Ad2 Bam HI C fragment (42.0 − 59.5 map units). All TK+ transformants contained Ad2 DNA as well as HSV-1 TK sequences. In most cases several inserts of virus DNA were present, many in high copy numbers. Although no Ad2 transcription promoter was present in the transforming DNA, Ad2-specific sequences were detected in polyadenylated cytoplasmic RNA species from several cell lines.
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ispartof Nucleic acids research, 1981-11, Vol.9 (21), p.5569-5585
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1362-4962
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_327544
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subjects Adenovirus
Adenoviruses, Human - enzymology
Adenoviruses, Human - genetics
Animals
Base Sequence
Cell Transformation, Viral
DNA, Viral - genetics
Herpes simplex virus 1
L Cells (Cell Line) - enzymology
Mice
Nucleic Acid Hybridization
Simplexvirus - enzymology
Simplexvirus - genetics
Thymidine Kinase - genetics
Transcription, Genetic
title Adenovirus late sequences linked to herpes simplex virus thymidine kinase may be introduced into eukaryotic cells and transcribed
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