A region of the polyoma virus genome between the replication origin and late protein coding sequences is required in cis for both early gene expression and viral DNA replication

Deletion mutants within the Py DNA region between the replication origin and the beginning of late protein coding sequences have been constructed and analysed for viability, early gene expression and viral DNA replication. Assay of replicative competence was facilitated by the use of Py transformed...

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Bibliographic Details
Published in:Nucleic acids research 1981-12, Vol.9 (23), p.6231-6250
Main Authors: Tyndall, Chiara, Mantia, Girolama La, Thacker, Colin M., Favaloro, Jennifer, Kamen, Robert
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Line
DNA Replication
DNA, Recombinant - metabolism
Genes, Viral
HeLa Cells
Humans
Mice
Mutation
Plasmids
Polyomavirus
Polyomavirus - genetics
Rats
Viral Proteins - genetics
Virus Replication
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Summary:Deletion mutants within the Py DNA region between the replication origin and the beginning of late protein coding sequences have been constructed and analysed for viability, early gene expression and viral DNA replication. Assay of replicative competence was facilitated by the use of Py transformed mouse cells (COP lines) which express functional large T-protein but contain no free viral DNA. Viable mutants defined three new nonessential regions of the genome. Certain deletions spanning the PvuII site at nt 5130 (67.4 mu) were unable to express early genes and had a cis-acting defect in DNA replication. Other mutants had intermediate phenotypes. Relevance of these results to eucaryotic “enhancer” elements is discussed.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/9.23.6231