Biosynthesis and transport of yeast mitochondrial phenylalanyl-tRNA synthetase

The biosynthesis of yeast mitochondrial Phe-tRNA synthetase is studied in vivo. Antibodies against the enzyme are raised in rabbits. They precipitate two proteins in the post-ribosomal supernatant of the yeast cell homogenate. Immunoprecipitate analysis on SDS - gel electrophoresls shows that the tw...

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Bibliographic Details
Published in:Nucleic acids research 1981-12, Vol.9 (23), p.6293-6304
Main Authors: Diatewa, Martin, Stahl, André J.C.
Format: Article
Language:English
Subjects:
Amino Acyl-tRNA Synthetases - metabolism
Cytosol - enzymology
Enzyme-Linked Immunosorbent Assay
Kinetics
Mitochondria - enzymology
Peptide Fragments - analysis
Phenylalanine-tRNA Ligase - biosynthesis
Phenylalanine-tRNA Ligase - metabolism
Saccharomyces cerevisiae - enzymology
Trypsin
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Summary:The biosynthesis of yeast mitochondrial Phe-tRNA synthetase is studied in vivo. Antibodies against the enzyme are raised in rabbits. They precipitate two proteins in the post-ribosomal supernatant of the yeast cell homogenate. Immunoprecipitate analysis on SDS - gel electrophoresls shows that the two types of mitochondrial enzyme subunits with molecular weights of 57,000 and 72,000, respectively, are cytoplasmically synthesized as larger, individual precursors. Terminal extensions of the precursors prevent enzyme activity. Mitochondrial membranes linked protease(s) play(s) an active role in maturation.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/9.23.6293