Some characteristics of apurinic sites in single- and double-stranded biologically active ϕX174 DNA

With biologically active DNA of the bacteriophage ϕX174, both single and double-stranded, some physico-chemical and biological parameters of the depurination reaction are studied. It is shown that in single-stranded DNA each apurinic site is lethal, while in double-stranded RFI-DNA only about 5% of...

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Bibliographic Details
Published in:Nucleic acids research 1981-12, Vol.9 (23), p.6591-6599
Main Authors: Lafleur, M.V.M., Woldhuis, J., Loman, H.
Format: Article
Language:English
Subjects:
Apurinic Acid
Bacteriophage phi X 174 - analysis
DNA, Single-Stranded
DNA, Viral
Hydrogen-Ion Concentration
Kinetics
phage Phi X174
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Summary:With biologically active DNA of the bacteriophage ϕX174, both single and double-stranded, some physico-chemical and biological parameters of the depurination reaction are studied. It is shown that in single-stranded DNA each apurinic site is lethal, while in double-stranded RFI-DNA only about 5% of these sites are lethal. Furthermore it is concluded that the apurinic sites are formed at different rates in single- and double-stranded DNA and also the conversion into breaks of the apurinic sites is different for both forms of DNA.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/9.23.6591