Antibiotic induced electrophoretic mobility shifts of DNA restriction fragments

Several antibiotics, netropsin, distamycin A, actinomycin D, Hoechst 33258 and olivomycin, which demonstrate base specificity in their DNA binding properties have been found to alter the electrophoretic mobility of DNA restriction fragments in native polyacrylamide gels. The antibiotics mostly reduc...

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Bibliographic Details
Published in:Nucleic acids research 1979, Vol.6 (5), p.1869-1879
Main Authors: Loucks, E, Chaconas, G, Blakesley, R W, Wells, R D, van de Sande, J H
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents
Chemical Phenomena
Chemistry
Dactinomycin
Distamycins
DNA Restriction Enzymes
DNA, Viral
Electrophoresis, Polyacrylamide Gel
Ethidium
Molecular Weight
Netropsin
Olivomycins
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Summary:Several antibiotics, netropsin, distamycin A, actinomycin D, Hoechst 33258 and olivomycin, which demonstrate base specificity in their DNA binding properties have been found to alter the electrophoretic mobility of DNA restriction fragments in native polyacrylamide gels. The antibiotics mostly reduced the migration of larger DNA fragments, but netropsin and Hoechst 33258 were observed to increase the migration rate of several DNA fragments of intermediate size. DNA fragments of similar molecular weight which comigrate as a single gel band can at times be separated as the result of differential mobility shifts promoted by antibiotic DNA complex formations.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/6.5.1869