Rapid isolation of long cDNA clones from existing libraries

Obtaining full-length or even near full-length cDNA clones has been a time-consuming and labor-intensive step in the analysis of cloned genes. Recent methods which use PCR as a preparative tool for cloning have made this step considerably more rapid, but may introduce sequence errors in the resultin...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1991-04, Vol.19 (8), p.1951-1952
Main Authors: HAMILTON, B. A, PALAZZOLO, M. J, MEYEROWITZ, E. M
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Biotechnology
Cloning, Molecular
DNA - isolation & purification
Drosophila melanogaster - genetics
Fundamental and applied biological sciences. Psychology
Gene Library
Genetic engineering
Genetic technics
Genetic Techniques
Methods. Procedures. Technologies
Molecular Sequence Data
Polymerase Chain Reaction
Synthetic digonucleotides and genes. Sequencing
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Obtaining full-length or even near full-length cDNA clones has been a time-consuming and labor-intensive step in the analysis of cloned genes. Recent methods which use PCR as a preparative tool for cloning have made this step considerably more rapid, but may introduce sequence errors in the resulting clones due to numerous sequential rounds of in vitro replication. We describe a method for identifying long cDNA clones from existing cDNA libraries using PCR purely as a diagnostic tool.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.8.1951