Effects of Invertebrate Iridescent Virus 6 in Phyllophaga vandinei and Its Potential as a Biocontrol Delivery System

Invertebrate iridescent virus 6 (IIV6) was determined to cause infection in Phyllophaga vandinei Smyth (Coleoptera: Scarabaeidae) through a range of modes of transmissions. This is the first evidence of IIV6 infection in P. vandinei that caused both patent and sub-lethal infections in larvae and adu...

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Bibliographic Details
Published in:Journal of insect science (Tucson, Ariz.) Ariz.), 2011, Vol.11 (44), p.1-10
Main Authors: Jenkins, DA, Hunter, WB, Goenaga, R
Format: Article
Language:English
Subjects:
Animals
beetle
Biological control
biological insecticide
Chilo iridescent virus
CIV
Coleoptera
Coleoptera - physiology
Coleoptera - virology
Development and progression
Disease transmission
entomopathogen
Feeding Behavior
IIV6
injection
insect virus
Iridovirus - physiology
Pest Control, Biological - methods
Physiological aspects
Sexual Behavior, Animal
virus delivery system
Virus diseases
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Summary:Invertebrate iridescent virus 6 (IIV6) was determined to cause infection in Phyllophaga vandinei Smyth (Coleoptera: Scarabaeidae) through a range of modes of transmissions. This is the first evidence of IIV6 infection in P. vandinei that caused both patent and sub-lethal infections in larvae and adults. Mortality rates were determined to be ∼30% when virus inoculum was injected into larvae or adults. Adults injected with virus showed dramatically altered behavior; injected beetles were not observed feeding or mating compared with adults injected with buffer or adults that were not injected. Tissue collected from infected adults resulted in infection when injected into healthy adults, as confirmed with PCR. PCR also confirmed that frass of infected larvae and adults contained virus, and when reconstituted frass from infected individuals was injected into healthy adults or larvae they become infected. Healthy adults could be infected by coming into contact with soil or plant material that had been exposed to infected adults as much as two weeks prior to introduction of nonvirus exposed adults. Although relatively low mortality resulted when adults or larvae were injected with the virus, the demonstration of horizontal transmission, potentially through frass of infected individuals, identifies a mode of transmission that may be exploited as a potential management tool to reduce P. vandinei.
ISSN:1536-2442
1536-2442
DOI:10.1673/031.011.0144