Proper regulation of Cdc42 activity is required for tight actin concentration at the equator during cytokinesis in adherent mammalian Cells

Cytokinesis in mammalian cells requires actin assembly at the equatorial region. Although functions of RhoA in this process have been well established, additional mechanisms are likely involved. We have examined if Cdc42 is involved in actin assembly during cytokinesis. Depletion of Cdc42 had no app...

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Bibliographic Details
Published in:Experimental cell research 2011-10, Vol.317 (16), p.2384-2389
Main Authors: Zhu, Xiaodong, Wang, Junxia, Moriguchi, Kazuki, Liow, Lu Ting, Ahmed, Sohail, Kaverina, Irina, Murata-Hori, Maki
Format: Article
Language:English
Subjects:
Actin
Actin Cytoskeleton - metabolism
Actin Cytoskeleton - pathology
Actins - genetics
Actins - metabolism
Adherent mammalian cells
Animals
Cdc42
cdc42 GTP-Binding Protein - deficiency
cdc42 GTP-Binding Protein - genetics
cdc42 GTP-Binding Protein - metabolism
Cell adhesion & migration
Cell Line
Cell Shape
Cellular biology
Cytokines
Cytokinesis
Cytokinesis - physiology
Epithelial Cells - cytology
Epithelial Cells - metabolism
Epithelial Cells - pathology
Gene expression
Kidney - cytology
Microscopy, Phase-Contrast
Mitosis - physiology
Rats
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
rhoA GTP-Binding Protein - metabolism
RNA, Small Interfering - genetics
Time-Lapse Imaging
Transfection
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Summary:Cytokinesis in mammalian cells requires actin assembly at the equatorial region. Although functions of RhoA in this process have been well established, additional mechanisms are likely involved. We have examined if Cdc42 is involved in actin assembly during cytokinesis. Depletion of Cdc42 had no apparent effects on the duration of cytokinesis, while overexpression of constitutively active Cdc42 (CACdc42) caused cytokinesis failure in normal rat kidney epithelial cells. Cells depleted of Cdc42 displayed abnormal cell morphology and caused a failure of tight accumulation of actin and RhoA at the equator. In contrast, in cells overexpressing CACdc42, actin formed abnormal bundles and RhoA was largely eliminated from the equator. Our results suggest that accurate regulation of Cdc42 activity is crucial for proper equatorial actin assembly and RhoA localization during cytokinesis. Notably, our observations also suggest that tight actin concentration is not essential for cytokinesis in adherent mammalian cells.
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2011.06.019