Proteomic analysis of stem cell differentiation and early development

Genomics methodologies have advanced to the extent that it is now possible to interrogate the gene expression in a single cell but proteomics has traditionally lagged behind and required much greater cellular input and was not quantitative. Coupling protein with gene expression data is essential for...

Full description

Saved in:
Bibliographic Details
Published in:Cold Spring Harbor perspectives in biology 2012-03, Vol.4 (3), p.a008177-a008177
Main Authors: van Hoof, Dennis, Krijgsveld, Jeroen, Mummery, Christine
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Cell Membrane - metabolism
Concepts
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Embryonic Stem Cells - physiology
Humans
Mass Spectrometry - methods
Mice
Phosphorylation
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - metabolism
Pluripotent Stem Cells - physiology
Protein Interaction Maps
Proteins - metabolism
Proteins - physiology
Proteomics - methods
Proteomics - trends
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Genomics methodologies have advanced to the extent that it is now possible to interrogate the gene expression in a single cell but proteomics has traditionally lagged behind and required much greater cellular input and was not quantitative. Coupling protein with gene expression data is essential for understanding how cell behavior is regulated. Advances primarily in mass spectrometry have, however, greatly improved the sensitivity of proteomics methods over the last decade and the outcome of proteomic analyses can now also be quantified. Nevertheless, it is still difficult to obtain sufficient tissue from staged mammalian embryos to combine proteomic and genomic analyses. Recent developments in pluripotent stem cell biology have in part addressed this issue by providing surrogate scalable cell systems in which early developmental events can be modeled. Here we present an overview of current proteomics methodologies and the kind of information this can provide on the biology of human and mouse pluripotent stem cells.
ISSN:1943-0264
1943-0264
DOI:10.1101/cshperspect.a008177