A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays

A procedure for immobilization of well-defined quantities of oligodeoxyribonucleotides (ODNs) to a versatile nylon support is described. The solid support, a nylon-6/6 bead, is covalently coated with poly(ethyleneimine) to provide a reactive spacer-arm for attachment of ODNs. 5'-Aminohexyl-tail...

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Bibliographic Details
Published in:Nucleic acids research 1991-06, Vol.19 (12), p.3345-3350
Main Authors: VAN NESS, J, KALBFLEISCH, S, PETRIE, C. R, REED, M. W, TABONE, J. C, VERMEULEN, N. M. J
Format: Article
Language:English
Subjects:
Alkylation
Base Sequence
Biological and medical sciences
Colorimetry
Diverse techniques
DNA
Fluorescence
Fundamental and applied biological sciences. Psychology
Genetic Techniques
Molecular and cellular biology
Molecular Sequence Data
Nucleic Acid Hybridization
Nylons
Oligonucleotide Probes
Polyethyleneimine
Triazines
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Description
Summary:A procedure for immobilization of well-defined quantities of oligodeoxyribonucleotides (ODNs) to a versatile nylon support is described. The solid support, a nylon-6/6 bead, is covalently coated with poly(ethyleneimine) to provide a reactive spacer-arm for attachment of ODNs. 5'-Aminohexyl-tailed ODNs are selectively activated using 2,4,6-trichloro-1,3,5-triazine (cyanuric chloride) and then covalently attached to the bead via the triazine moiety. The modified nylon support has a low level of binding of nonspecific nucleic acid and efficiently captures both RNA and DNA targets.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.12.3345