Random-primed cDNA synthesis facilitates the isolation of multiple 5'-cDNA ends by RACE

The RACE (rapid amplification of cDNA ends) technique can be used to amplify 5'- and 3'-cDNA ends that derive from transcripts of low abundance. To isolate the 5' end of a specific cDNA (5' RACE), a small, anti-sense, transcript-specific oligonucleotide is used to prime first-str...

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Bibliographic Details
Published in:Nucleic acids research 1991-07, Vol.19 (14), p.4002-4002
Main Authors: HARVEY, R. J, DARLISON, M. G
Format: Article
Language:English
Subjects:
Animals
Apicomplexa - genetics
Biological and medical sciences
Biotechnology
DNA, Protozoan - chemical synthesis
DNA, Protozoan - isolation & purification
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Molecular cloning
Nucleic Acid Amplification Techniques
Templates, Genetic
Citations: Items that cite this one
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Summary:The RACE (rapid amplification of cDNA ends) technique can be used to amplify 5'- and 3'-cDNA ends that derive from transcripts of low abundance. To isolate the 5' end of a specific cDNA (5' RACE), a small, anti-sense, transcript-specific oligonucleotide is used to prime first-strand cDNA synthesis. The specific first-strand cDNA is then purified, and polyadenylated using terminal deoxynucleotidyl transferase (TdT) and dATP. We have successfully used random-primed, polyadenylated, first-strand cDNA in combination with 5' RACE.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.14.4002