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Evaluation of Measles Vaccine Virus as a Vector to Deliver Respiratory Syncytial Virus Fusion Protein or Epstein-Barr Virus Glycoprotein gp350
Live attenuated recombinant measles vaccine virus (MV) Edmonston-Zagreb (EZ) strain was evaluated as a viral vector to express the ectodomains of fusion protein of respiratory syncytial virus (RSV F) or glycoprotein 350 of Epstein-Barr virus (EBV gp350) as candidate vaccines for prophylaxis of RSV a...
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Published in: | The open virology journal 2012, Vol.6 (1), p.12-22 |
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description | Live attenuated recombinant measles vaccine virus (MV) Edmonston-Zagreb (EZ) strain was evaluated as a viral vector to express the ectodomains of fusion protein of respiratory syncytial virus (RSV F) or glycoprotein 350 of Epstein-Barr virus (EBV gp350) as candidate vaccines for prophylaxis of RSV and EBV. The glycoprotein gene was inserted at the 1(st) or the 3(rd) position of the measles virus genome and the recombinant viruses were generated. Insertion of the foreign gene at the 3(rd) position had a minimal impact on viral replication in vitro. RSV F or EBV gp350 protein was secreted from infected cells. In cotton rats, EZ-RSV F and EZ-EBV gp350 induced MV- and insert-specific antibody responses. In addition, both vaccines also induced insert specific interferon gamma (IFN-γ) secreting T cell response. EZ-RSV F protected cotton rats from pulmonary replication of RSV A2 challenge infection. In rhesus macaques, although both EZ-RSV F and EZ-EBV gp350 induced MV specific neutralizing antibody responses, only RSV F specific antibody response was detected. Thus, the immunogenicity of the foreign antigens delivered by measles vaccine virus is dependent on the nature of the insert and the animal models used for vaccine evaluation. |
doi_str_mv | 10.2174/1874357901206010012 |
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The glycoprotein gene was inserted at the 1(st) or the 3(rd) position of the measles virus genome and the recombinant viruses were generated. Insertion of the foreign gene at the 3(rd) position had a minimal impact on viral replication in vitro. RSV F or EBV gp350 protein was secreted from infected cells. In cotton rats, EZ-RSV F and EZ-EBV gp350 induced MV- and insert-specific antibody responses. In addition, both vaccines also induced insert specific interferon gamma (IFN-γ) secreting T cell response. EZ-RSV F protected cotton rats from pulmonary replication of RSV A2 challenge infection. In rhesus macaques, although both EZ-RSV F and EZ-EBV gp350 induced MV specific neutralizing antibody responses, only RSV F specific antibody response was detected. 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The glycoprotein gene was inserted at the 1(st) or the 3(rd) position of the measles virus genome and the recombinant viruses were generated. Insertion of the foreign gene at the 3(rd) position had a minimal impact on viral replication in vitro. RSV F or EBV gp350 protein was secreted from infected cells. In cotton rats, EZ-RSV F and EZ-EBV gp350 induced MV- and insert-specific antibody responses. In addition, both vaccines also induced insert specific interferon gamma (IFN-γ) secreting T cell response. EZ-RSV F protected cotton rats from pulmonary replication of RSV A2 challenge infection. In rhesus macaques, although both EZ-RSV F and EZ-EBV gp350 induced MV specific neutralizing antibody responses, only RSV F specific antibody response was detected. Thus, the immunogenicity of the foreign antigens delivered by measles vaccine virus is dependent on the nature of the insert and the animal models used for vaccine evaluation.</description><subject>Animal models</subject><subject>Antibody response</subject><subject>Epstein-Barr virus</subject><subject>Fusion protein</subject><subject>gamma -Interferon</subject><subject>Genomes</subject><subject>Glycoproteins</subject><subject>Immunogenicity</subject><subject>Infection</subject><subject>Insertion</subject><subject>Lung</subject><subject>Lymphocytes T</subject><subject>Macaca mulatta</subject><subject>Measles virus</subject><subject>Prophylaxis</subject><subject>Replication</subject><subject>Respiratory syncytial virus</subject><subject>Vaccines</subject><issn>1874-3579</issn><issn>1874-3579</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kd1qFTEUhYNYbK0-gSC505vR_M1M5kbQelqFloo_5zbsZnZqJGcyJjMH5iV8ZmfsWCoUIbA3K99aJCxCnnH2SvBavea6VrKsG8YFqxhn83xAjha1WOSHd_ZD8jjnH4xVQmn-iBwKIbVsWHVEfm32EEYYfOxodPQCIQfMdAvW-g7p1qcxU5gP3aIdYqJDpO8x-D0m-hlz7xPM6kS_TJ2dBg9htZyOeYn8lOKAfo5OdNPnZS3eQUordBYmG_sVue5lyZ6QAwch49N1HpNvp5uvJx-K88uzjydvzwsrSyEKXYmGKZCaqSvuWkDdaLCuBsVkjYI1lkNr0XElRa1d6xRwVyK0pWyZaq08Jm9ucvvxaocz2g0JgumT30GaTARv_r3p_HdzHfdGCl1pxeeAF2tAij9HzIPZ-WwxBOgwjtk0ouKlYFLO5Mv_klzysuKV_oPKG9SmmHNCd_sgzszSubmn89n1_O5fbj1_S5a_AXFpqbI</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Mok, Hoyin</creator><creator>Cheng, Xing</creator><creator>Xu, Qi</creator><creator>Zengel, James R</creator><creator>Parhy, Bandita</creator><creator>Zhao, Jackie</creator><creator>Wang, C Kathy</creator><creator>Jin, Hong</creator><general>Bentham Open</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2012</creationdate><title>Evaluation of Measles Vaccine Virus as a Vector to Deliver Respiratory Syncytial Virus Fusion Protein or Epstein-Barr Virus Glycoprotein gp350</title><author>Mok, Hoyin ; Cheng, Xing ; Xu, Qi ; Zengel, James R ; Parhy, Bandita ; Zhao, Jackie ; Wang, C Kathy ; Jin, Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3522-862904a3804b1fdae898acf7a4037e209c1adcef143278fdf4a1f5ead53d04dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animal models</topic><topic>Antibody response</topic><topic>Epstein-Barr virus</topic><topic>Fusion protein</topic><topic>gamma -Interferon</topic><topic>Genomes</topic><topic>Glycoproteins</topic><topic>Immunogenicity</topic><topic>Infection</topic><topic>Insertion</topic><topic>Lung</topic><topic>Lymphocytes T</topic><topic>Macaca mulatta</topic><topic>Measles virus</topic><topic>Prophylaxis</topic><topic>Replication</topic><topic>Respiratory syncytial virus</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mok, Hoyin</creatorcontrib><creatorcontrib>Cheng, Xing</creatorcontrib><creatorcontrib>Xu, Qi</creatorcontrib><creatorcontrib>Zengel, James R</creatorcontrib><creatorcontrib>Parhy, Bandita</creatorcontrib><creatorcontrib>Zhao, Jackie</creatorcontrib><creatorcontrib>Wang, C Kathy</creatorcontrib><creatorcontrib>Jin, Hong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The open virology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mok, Hoyin</au><au>Cheng, Xing</au><au>Xu, Qi</au><au>Zengel, James R</au><au>Parhy, Bandita</au><au>Zhao, Jackie</au><au>Wang, C Kathy</au><au>Jin, Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Measles Vaccine Virus as a Vector to Deliver Respiratory Syncytial Virus Fusion Protein or Epstein-Barr Virus Glycoprotein gp350</atitle><jtitle>The open virology journal</jtitle><addtitle>Open Virol J</addtitle><date>2012</date><risdate>2012</risdate><volume>6</volume><issue>1</issue><spage>12</spage><epage>22</epage><pages>12-22</pages><issn>1874-3579</issn><eissn>1874-3579</eissn><abstract>Live attenuated recombinant measles vaccine virus (MV) Edmonston-Zagreb (EZ) strain was evaluated as a viral vector to express the ectodomains of fusion protein of respiratory syncytial virus (RSV F) or glycoprotein 350 of Epstein-Barr virus (EBV gp350) as candidate vaccines for prophylaxis of RSV and EBV. 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subjects | Animal models Antibody response Epstein-Barr virus Fusion protein gamma -Interferon Genomes Glycoproteins Immunogenicity Infection Insertion Lung Lymphocytes T Macaca mulatta Measles virus Prophylaxis Replication Respiratory syncytial virus Vaccines |
title | Evaluation of Measles Vaccine Virus as a Vector to Deliver Respiratory Syncytial Virus Fusion Protein or Epstein-Barr Virus Glycoprotein gp350 |
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