Model System for Cell Adhesion Mediated by Weak Carbohydrate–Carbohydrate Interactions

The multivalent carbohydrate–carbohydrate interaction between membrane-anchored epitopes derived from the marine sponge Microciona prolifera has been explored by colloidal probe microscopy. An in situ coupling of sulfated and non-sulfated disaccharides to membrane-coated surfaces was employed to mim...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2012-02, Vol.134 (7), p.3326-3329
Main Authors: Lorenz, Bärbel, Álvarez de Cienfuegos, Luis, Oelkers, Marieelen, Kriemen, Ella, Brand, Christian, Stephan, Milena, Sunnick, Eva, Yüksel, Deniz, Kalsani, Venkateshwarlu, Kumar, Krishna, Werz, Daniel B, Janshoff, Andreas
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion
disaccharides
Disaccharides - chemistry
Disaccharides - isolation & purification
epitopes
ions
Lipid Bilayers - chemistry
microscopy
Microscopy, Scanning Probe
Porifera
Porifera - chemistry
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Summary:The multivalent carbohydrate–carbohydrate interaction between membrane-anchored epitopes derived from the marine sponge Microciona prolifera has been explored by colloidal probe microscopy. An in situ coupling of sulfated and non-sulfated disaccharides to membrane-coated surfaces was employed to mimic native cell–cell contacts. The dynamic strength of the homomeric self-association was measured as a function of calcium ions and loading rate. A deterministic model was used to estimate the basic energy landscape and number of participating bonds in the contact zone.
ISSN:0002-7863
1520-5126
1520-5126
DOI:10.1021/ja210304j