A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies

In genetic linkage studies using the restriction fragment length polymorphism (RFLP) technique, it is essential to process effectively large numbers of blood samples. The method described avoids the use of any organic solvents. This is achieved by salting out the cellular proteins by dehydration and...

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Bibliographic Details
Published in:Nucleic acids research 1991-10, Vol.19 (19), p.5444-5444
Main Authors: LAHIRI, D. K, NURNBERGER, J. I
Format: Article
Language:English
Subjects:
Biological and medical sciences
blood
Centrifugation
Chemical Precipitation
Diverse techniques
DNA - blood
DNA - isolation & purification
Fundamental and applied biological sciences. Psychology
Humans
Methods
Molecular and cellular biology
Polymorphism, Restriction Fragment Length
Sodium Chloride - chemistry
Sodium Dodecyl Sulfate - chemistry
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Summary:In genetic linkage studies using the restriction fragment length polymorphism (RFLP) technique, it is essential to process effectively large numbers of blood samples. The method described avoids the use of any organic solvents. This is achieved by salting out the cellular proteins by dehydration and precipitation with a saturated sodium chloride solution. Most of the procedures also involve prolonged incubation with proteinase K. Our procedure eliminates completely the use of proteinase K treatment.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.19.5444