Effect of Inducers, Incubation Time and Heme Concentration on IC50 Value Variation in Anti-heme Crystallization Assay

Heme detoxification through crystallization into hemozoin has been suggested as a good target for the development of screening assays for new antimalarials. However, comparisons among the data obtained from different experiments are difficult, and the IC50 values (the concentrations of drug that are...

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Bibliographic Details
Published in:Tropical Medicine and Health 2011, Vol.39(4), pp.119-126
Main Authors: Nhien, Nguyen Thanh Thuy, Huy, Nguyen Tien, Uyen, Dinh Thanh, Deharo, Eric, Hoa, Pham Thi Le, Hirayama, Kenji, Harada, Shigeharu, Kamei, Kaeko
Format: Article
Language:English
Subjects:
antimalarial
heme crystallization
hemozoin
IC50
inducer
Original
β-hematin
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Summary:Heme detoxification through crystallization into hemozoin has been suggested as a good target for the development of screening assays for new antimalarials. However, comparisons among the data obtained from different experiments are difficult, and the IC50 values (the concentrations of drug that are required to inhibit 50% of hemozoin formation) for the same drug vary widely. We studied the effects of changes in heme concentration (precursor of β-hematin), incubation time and three inducers (SDS, Tween 20 and linoleic acid) on the IC50 of some antimalarials (chloroquine, quinine, amodiaquine, and clotrimazole). The results showed that increasing both inducer concentration and incubation time raised the IC50 of selected antimalarials. Any change in those factors caused the IC50 value to vary. Standardization of assay conditions is, therefore, necessary to increase reproducibility and reduce discrepancies in assay performance. Considering all of the variables, the best choice of inducers is in the order of SDS > Tween 20 > linoleic acid.
ISSN:1348-8945
1349-4147
DOI:10.2149/tmh.2011-29