W (A or T) sequences as probes and primers suitable for genomic mapping and fingerprinting

A limitation to the use of oligonucleotide probes as tools for genetic and physical mapping has been the low hybridization positive frequency obtained by oligonucleotides of sufficient length to hybridize preferentially to cloned insert DNA (and not host E. coli genomic DNA). Both computer and exper...

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Bibliographic Details
Published in:Nucleic acids research 1991-11, Vol.19 (21), p.5839-5842
Main Authors: DRMANAC, R, NIZETIC, D, LENNON, G. G, BEITVERDA, A, LEHRACH, H
Format: Article
Language:English
Subjects:
Adenine
Base Sequence
Biological and medical sciences
Chromosome Mapping - methods
Cloning, Molecular
Cosmids - genetics
DNA Fingerprinting - methods
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genes. Genome
Humans
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Hybridization - genetics
oligodeoxyribonucleotides
Oligonucleotide Probes - genetics
Thymine
X Chromosome
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Summary:A limitation to the use of oligonucleotide probes as tools for genetic and physical mapping has been the low hybridization positive frequency obtained by oligonucleotides of sufficient length to hybridize preferentially to cloned insert DNA (and not host E. coli genomic DNA). Both computer and experimental results now indicate that oligonucleotide probes composed of W (A or T) sequence are preferentially found in eukaryotic DNA, and can be used to provide high frequency, discriminative hybridization. Such W sequences may be useful as either probes or PCR primers in molecular diagnostic applications as well as in genetic and physical mapping.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.21.5839