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W (A or T) sequences as probes and primers suitable for genomic mapping and fingerprinting

A limitation to the use of oligonucleotide probes as tools for genetic and physical mapping has been the low hybridization positive frequency obtained by oligonucleotides of sufficient length to hybridize preferentially to cloned insert DNA (and not host E. coli genomic DNA). Both computer and exper...

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Published in:	Nucleic acids research 1991-11, Vol.19 (21), p.5839-5842
Main Authors:	DRMANAC, R, NIZETIC, D, LENNON, G. G, BEITVERDA, A, LEHRACH, H
Format:	Article
Language:	English
Subjects:	Adenine Base Sequence Biological and medical sciences Chromosome Mapping - methods Cloning, Molecular Cosmids - genetics DNA Fingerprinting - methods Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes. Genome Humans Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Hybridization - genetics oligodeoxyribonucleotides Oligonucleotide Probes - genetics Thymine X Chromosome
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creator	DRMANAC, R NIZETIC, D LENNON, G. G BEITVERDA, A LEHRACH, H
description	A limitation to the use of oligonucleotide probes as tools for genetic and physical mapping has been the low hybridization positive frequency obtained by oligonucleotides of sufficient length to hybridize preferentially to cloned insert DNA (and not host E. coli genomic DNA). Both computer and experimental results now indicate that oligonucleotide probes composed of W (A or T) sequence are preferentially found in eukaryotic DNA, and can be used to provide high frequency, discriminative hybridization. Such W sequences may be useful as either probes or PCR primers in molecular diagnostic applications as well as in genetic and physical mapping.
doi_str_mv	10.1093/nar/19.21.5839
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subjects	Adenine Base Sequence Biological and medical sciences Chromosome Mapping - methods Cloning, Molecular Cosmids - genetics DNA Fingerprinting - methods Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes. Genome Humans Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Hybridization - genetics oligodeoxyribonucleotides Oligonucleotide Probes - genetics Thymine X Chromosome
title	W (A or T) sequences as probes and primers suitable for genomic mapping and fingerprinting
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