Promoter architecture of mouse olfactory receptor genes

Odorous chemicals are detected by the mouse main olfactory epithelium (MOE) by about 1100 types of olfactory receptors (OR) expressed by olfactory sensory neurons (OSNs). Each mature OSN is thought to express only one allele of a single OR gene. Major impediments to understand the transcriptional co...

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Bibliographic Details
Published in:Genome research 2012-03, Vol.22 (3), p.486-497
Main Authors: Plessy, Charles, Pascarella, Giovanni, Bertin, Nicolas, Akalin, Altuna, Carrieri, Claudia, Vassalli, Anne, Lazarevic, Dejan, Severin, Jessica, Vlachouli, Christina, Simone, Roberto, Faulkner, Geoffrey J, Kawai, Jun, Daub, Carsten O, Zucchelli, Silvia, Hayashizaki, Yoshihide, Mombaerts, Peter, Lenhard, Boris, Gustincich, Stefano, Carninci, Piero
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Animals
Base Sequence
Binding Sites
Consensus Sequence
DNA-Binding Proteins - metabolism
Gene Expression Profiling
Gene Expression Regulation
Gene Order
Genetic Loci
MEF2 Transcription Factors
Mice
Mice, Transgenic
Myogenic Regulatory Factors - metabolism
Olfactory Mucosa - metabolism
Olfactory Receptor Neurons - metabolism
Promoter Regions, Genetic
Receptors, Odorant - genetics
TATA-Box Binding Protein - metabolism
Transcription Factors - metabolism
Transcription Initiation Site
Transcription, Genetic
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Summary:Odorous chemicals are detected by the mouse main olfactory epithelium (MOE) by about 1100 types of olfactory receptors (OR) expressed by olfactory sensory neurons (OSNs). Each mature OSN is thought to express only one allele of a single OR gene. Major impediments to understand the transcriptional control of OR gene expression are the lack of a proper characterization of OR transcription start sites (TSSs) and promoters, and of regulatory transcripts at OR loci. We have applied the nanoCAGE technology to profile the transcriptome and the active promoters in the MOE. nanoCAGE analysis revealed the map and architecture of promoters for 87.5% of the mouse OR genes, as well as the expression of many novel noncoding RNAs including antisense transcripts. We identified candidate transcription factors for OR gene expression and among them confirmed by chromatin immunoprecipitation the binding of TBP, EBF1 (OLF1), and MEF2A to OR promoters. Finally, we showed that a short genomic fragment flanking the major TSS of the OR gene Olfr160 (M72) can drive OSN-specific expression in transgenic mice.
ISSN:1088-9051
1549-5469
DOI:10.1101/gr.126201.111