Insulin resistance is associated with a modest increase in inflammation in subcutaneous adipose tissue of moderately obese women

• Published in: Diabetologia 2008-12, Vol.51 (12), p.2303-2308
• Main Authors: McLaughlin, T, Deng, A, Gonzales, O, Aillaud, M, Yee, G, Lamendola, C, Abbasi, F, Connolly, A. J, Sherman, A, Cushman, S. W, Reaven, G, Tsao, P. S
• Format: Article
• Language: English
• Subjects: Abdomen; Adipocyte; Adipocytes; adipose tissue; Adult; Aged; Biological and medical sciences; Body fat; Diabetes; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Female; Gastrointestinal surgery; Gene expression; Gene Expression Regulation; Glucose; Human Physiology; Humans; Inflammation; Inflammation Mediators - metabolism; Insulin Resistance; Internal Medicine; Kidney diseases; Leukocyte Common Antigens - metabolism; Medical sciences; Medicine; Medicine & Public Health; Metabolic Diseases; Middle Aged; Obesity; Obesity - genetics; Obesity - metabolism; Overweight; Plasma; Subcutaneous Fat - metabolism; Weight control; Womens health
• ISSN: 0012-186X; 1432-0428
• DOI: 10.1007/s00125-008-1148-z

Aims/hypothesis We have previously described differences in adipose cell size distribution and expression of genes related to adipocyte differentiation in subcutaneous abdominal fat obtained from insulin-sensitive (IS) and -resistant (IR) persons, matched for degree of moderate obesity. To determine whether other biological properties also differ between IR and IS obese individuals, we quantified markers of inflammatory activity in adipose tissue from overweight IR and IS individuals. Methods Subcutaneous abdominal tissue was obtained from moderately obese women, divided into IR (n = 14) and IS (n = 19) subgroups by determining their steady-state plasma glucose (SSPG) concentrations during the insulin suppression test. Inflammatory activity was assessed by comparing expression of nine relevant genes and by immunohistochemical quantification of CD45- and CD68-containing cells. Results SSPG concentrations were approximately threefold higher in IR than in IS individuals. Expression levels of CD68, EMR1, IL8, IL6 and MCP/CCL2 mRNAs were modestly but significantly increased (p < 0.05) in IR compared with IS participants. Results of immunohistochemical staining were consistent with gene expression data, demonstrating modest differences between IR and IS individuals. Crown-like structures, in which macrophages surround single adipocytes, were rarely seen in tissue from either subgroup. Conclusions/interpretation A modest increase in inflammatory activity was seen in subcutaneous adipose tissue from IR compared with equally obese IS individuals. Together with previous evidence of impaired adipose cell differentiation in IR vs equally obese individuals, it appears that at least two biological processes in subcutaneous adipose tissue characterize the insulin-resistant state independent of obesity per se. Trial registration ClinicalTrials.gov NCT00285844 Funding The study was funded by grants from the National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) and by the NIDDK Intramural Research Program.