A General RNA Motif for Cellular Transfection

We have developed a selection scheme to generate nucleic acid sequences that recognize and directly internalize into mammalian cells without the aid of conventional delivery methods. To demonstrate the generality of the technology, two independent selections with different starting pools were perfor...

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Bibliographic Details
Published in:Molecular therapy 2012-03, Vol.20 (3), p.616-624
Main Authors: Magalhães, Maria LB, Byrom, Michelle, Yan, Amy, Kelly, Linsley, Li, Na, Furtado, Raquel, Palliser, Deborah, Ellington, Andrew D, Levy, Matthew
Format: Article
Language:English
Subjects:
Acids
Animals
Antigens
Base Sequence
Biochemistry
Cell Line
Cells
Endocytosis
Epidermal growth factor
Female
Flow Cytometry
Gene Library
Gene Transfer Techniques
Humans
Libraries
Male
Medical research
Mice
Mice, Inbred BALB C
Mucous Membrane - metabolism
Nucleic Acid Conformation
Nucleotide Motifs
Original
Prostate
RNA - chemistry
Transfection
Vagina - metabolism
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Summary:We have developed a selection scheme to generate nucleic acid sequences that recognize and directly internalize into mammalian cells without the aid of conventional delivery methods. To demonstrate the generality of the technology, two independent selections with different starting pools were performed against distinct target cells. Each selection yielded a single highly functional sequence, both of which folded into a common core structure. This internalization signal can be adapted for use as a general purpose reagent for transfection into a wide variety of cell types including primary cells.
ISSN:1525-0016
1525-0024
DOI:10.1038/mt.2011.277