Activated STAT1 Transcription Factors Conduct Distinct Saltatory Movements in the Cell Nucleus

The activation of STAT transcription factors is a critical determinant of their subcellular distribution and their ability to regulate gene expression. Yet, it is not known how activation affects the behavior of individual STAT molecules in the cytoplasm and nucleus. To investigate this issue, we in...

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Bibliographic Details
Published in:Biophysical journal 2011-12, Vol.101 (11), p.2592-2600
Main Authors: Speil, Jasmin, Baumgart, Eugen, Siebrasse, Jan-Peter, Veith, Roman, Vinkemeier, Uwe, Kubitscheck, Ulrich
Format: Article
Language:English
Subjects:
Cell Nucleus - metabolism
Cell Survival
Cell Tracking
Cells
Cellular Biophysics and Electrophysiology
Chromatin
Cytoplasm
Cytosol - metabolism
Fluorescent Dyes - metabolism
Gene expression
HeLa Cells
Humans
Microscopy
Molecules
Mutant Proteins - metabolism
Protein Transport
STAT1 Transcription Factor - chemistry
STAT1 Transcription Factor - metabolism
transcription (genetics)
transcription factors
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Summary:The activation of STAT transcription factors is a critical determinant of their subcellular distribution and their ability to regulate gene expression. Yet, it is not known how activation affects the behavior of individual STAT molecules in the cytoplasm and nucleus. To investigate this issue, we injected fluorescently labeled STAT1 in living HeLa cells and traced them by single-molecule microscopy. We determined that STAT1 moved stochastically in the cytoplasm and nucleus with very short residence times (
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2011.10.006