Cloning of cDNA encoding the complete precursor for bovine seminal ribonuclease

Palmieri et al. cloned and sequenced a cDNA fragment encoding bovine seminal ribonuclease (RNase BS1) from amino acid residue 47 up to the C-terminal residue. On screening a cDNA library from bovine seminal vesicle tissue by colony hybridisation employing synthetic primers a number of RNase BS1-spec...

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Bibliographic Details
Published in:Nucleic acids research 1990-02, Vol.18 (4), p.1057-1057
Main Authors: PREUSS, K.-D, WAGNER, S, FREUDENSTEIN, J, HEINZ SCHEIT, K
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Cattle
Cloning, Molecular
DNA - genetics
Enzyme Precursors - genetics
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
Male
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
pancreatic ribonuclease
Ribonucleases - genetics
Semen - enzymology
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Summary:Palmieri et al. cloned and sequenced a cDNA fragment encoding bovine seminal ribonuclease (RNase BS1) from amino acid residue 47 up to the C-terminal residue. On screening a cDNA library from bovine seminal vesicle tissue by colony hybridisation employing synthetic primers a number of RNase BS1-specific clones were identified. cDNA clone pBSV5 displays an open reading frame encoding a hydrophobic leader peptide consisting of 26 aa and the mature RNase BS1 of 124 aa. pBSV5 comprises the full 3'-untranslated region.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.4.1057