The chorion genes of the medfly, Ceratitis capitata. I : Structural and regulatory conservation of the s36 gene relative to two Drosophila species

We have used low stringency screening with the Drosophila melanogaster s36 chorion gene to recover its homologue from genomic and cDNA libraries of the medfly, Ceratitis capitata. The same gene has also been recovered from a genomic library of D. virilis. The medfly s36 gene shows similar developmen...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1990-04, Vol.18 (7), p.1731-1737
Main Authors: KONSOLAKI, M, KOMITOPOULOU, K, TOLIAS, P. P, KING, D. L, SWIMMER, C, KAFATOS, F. C
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Ceratitis capitata
Chorion
Diptera
Diptera - genetics
Drosophila - genetics
Drosophila melanogaster
Fundamental and applied biological sciences. Psychology
Genes
Genes. Genome
Genomic Library
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Hybridization
Restriction Mapping
RNA - genetics
RNA - isolation & purification
Sequence Homology, Nucleic Acid
Tephritidae
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have used low stringency screening with the Drosophila melanogaster s36 chorion gene to recover its homologue from genomic and cDNA libraries of the medfly, Ceratitis capitata. The same gene has also been recovered from a genomic library of D. virilis. The medfly s36 gene shows similar developmental specificity as in Drosophila (early choriogenesis). It is also specifically amplified in ovarian follicles; this is the first report of chorion gene amplification outside the genus Drosophila. Alignments of s36 sequences from three species show that, in addition to its regulatory conservation, the s36 gene is extensively conserved in sequence, in a region corresponding to a central protein domain, and in short regions of 5' flanking DNA that might correspond to cis-regulatory elements.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.7.1731