Regulatory elements in the first intron contribute to transcriptional regulation of the beta 3 tubulin gene by 20-hydroxyecdysone in Drosophila Kc cells

We have studied the transcriptional regulation of the beta 3 tubulin gene by the steroid hormone 20-hydroxyecdysone (20-OH-E) in Drosophila Kc cells. A series of hybrid genes with varying tubulin gene lengths driving the bacterial chloramphenicol acetyl transferase (CAT) gene were constructed. The p...

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Bibliographic Details
Published in:Nucleic acids research 1990-05, Vol.18 (10), p.2861-2867
Main Authors: Bruhat, A, Tourmente, S, Chapel, S, Sobrier, M L, Couderc, J L, Dastugue, B
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cloning, Molecular
Drosophila
Drosophila - genetics
Ecdysterone - pharmacology
Gene Expression Regulation - drug effects
Genes
Introns
Life Sciences
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid
Transcription, Genetic - drug effects
Tubulin - genetics
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Summary:We have studied the transcriptional regulation of the beta 3 tubulin gene by the steroid hormone 20-hydroxyecdysone (20-OH-E) in Drosophila Kc cells. A series of hybrid genes with varying tubulin gene lengths driving the bacterial chloramphenicol acetyl transferase (CAT) gene were constructed. The promoter activity was assayed after transient expression in Kc cells, in the presence or absence of 20-OH-E. We find that 0.91Kb upstream from the transcription start site contain one or several hormone independent positive cis-acting elements, responsible for the constitutive expression of the beta 3 tubulin gene. In the large (4.5 Kb) first intron of this gene, we identified additional hormone dependent negative and positive regulatory elements, which can act in both directions and in a position-independence manner. Then, the negative intron element(s), which repress the transcription in the absence of 20-OH-E has characteristics of silencer.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.10.2861