Significance of Xenobiotic Metabolism for Bioaccumulation Kinetics of Organic Chemicals in Gammarus pulex

• Published in: Environmental science & technology 2012-03, Vol.46 (6), p.3498-3508
• Main Authors: Ashauer, Roman, Hintermeister, Anita, O’Connor, Isabel, Elumelu, Maline, Hollender, Juliane, Escher, Beate I
• Format: Article
• Language: English
• Subjects: Amphipoda - metabolism; Animal, plant and microbial ecology; Animals; Applied ecology; Bioaccumulation; Biological and medical sciences; Biotransformation; Chemical compounds; Crustaceans; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on protozoa and invertebrates; Fundamental and applied biological sciences. Psychology; Kinetics; Mass spectrometry; Metabolites; Models, Biological; Organic chemicals; Organic Chemicals - pharmacokinetics; Toxicity; Water Pollutants, Chemical - pharmacokinetics; Xenobiotics - pharmacokinetics
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es204611h

Bioaccumulation and biotransformation are key toxicokinetic processes that modify toxicity of chemicals and sensitivity of organisms. Bioaccumulation kinetics vary greatly among organisms and chemicals; thus, we investigated the influence of biotransformation kinetics on bioaccumulation in a model aquatic invertebrate using fifteen 14C-labeled organic xenobiotics from diverse chemical classes and physicochemical properties (1,2,3-trichlorobenzene, imidacloprid, 4,6-dinitro-o-cresol, ethylacrylate, malathion, chlorpyrifos, aldicarb, carbofuran, carbaryl, 2,4-dichlorophenol, 2,4,5-trichlorophenol, pentachlorophenol, 4-nitrobenzyl-chloride, 2,4-dichloroaniline, and sea-nine (4,5-dichloro-2-octyl-3-isothiazolone)). We detected and identified metabolites using HPLC with UV and radio-detection as well as high resolution mass spectrometry (LTQ-Orbitrap). Kinetics of uptake, biotransformation, and elimination of parent compounds and metabolites were modeled with a first-order one-compartment model. Bioaccumulation factors were calculated for parent compounds and metabolite enrichment factors for metabolites. Out of 19 detected metabolites, we identified seven by standards or accurate mass measurements and two via pathway analysis and analogies to other compounds. 1,2,3-Trichlorobenzene, imidacloprid, and 4,6-dinitro-o-cresol were not biotransformed. Dietary uptake contributed little to overall uptake. Differentiation between parent and metabolites increased accuracy of bioaccumulation parameters compared to total 14C measurements. Biotransformation dominated toxicokinetics and strongly affected internal concentrations of parent compounds and metabolites. Many metabolites reached higher internal concentrations than their parents, characterized by large metabolite enrichment factors.