Abundance of Prereplicative Complexes (Pre-RCs) Facilitates Recombinational Repair under Replication Stress in Fission Yeast

• Published in: The Journal of biological chemistry 2011-12, Vol.286 (48), p.41701-41710
• Main Authors: Maki, Kentaro, Inoue, Takahiro, Onaka, Atsushi, Hashizume, Hiroko, Somete, Naoko, Kobayashi, Yuko, Murakami, Shigefumi, Shigaki, Chikako, Takahashi, Tatsuro S., Masukata, Hisao, Nakagawa, Takuro
• Format: Article
• Language: English
• Subjects: Cdt1; DNA and Chromosomes; DNA Damage Response; DNA Recombination; DNA Repair; DNA Replication; DNA, Fungal - biosynthesis; DNA, Fungal - genetics; G1 Phase - physiology; Gene Expression Regulation, Fungal - physiology; Genomic Instability; Mcm6; Multiprotein Complexes - genetics; Multiprotein Complexes - metabolism; Mutation; Phosphorylation; Replication Stress; Rhp54; S Phase - physiology; Schizosaccharomyces - genetics; Schizosaccharomyces - metabolism; Schizosaccharomyces pombe Proteins - genetics; Schizosaccharomyces pombe Proteins - metabolism
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M111.285619

Mcm2–7 complexes are loaded onto chromatin with the aid of Cdt1 and Cdc18/Cdc6 and form prereplicative complexes (pre-RCs) at multiple sites on each chromosome. Pre-RCs are essential for DNA replication and surviving replication stress. However, the mechanism by which pre-RCs contribute to surviving replication stress is largely unknown. Here, we isolated the fission yeast mcm6-S1 mutant that was hypersensitive to methyl methanesulfonate (MMS) and camptothecin (CPT), both of which cause forks to collapse. The mcm6-S1 mutation impaired the interaction with Cdt1 and decreased the binding of minichromosome maintenance (MCM) proteins to replication origins. Overexpression of Cdt1 restored MCM binding and suppressed the sensitivity to MMS and CPT, suggesting that the Cdt1-Mcm6 interaction is important for the assembly of pre-RCs and the repair of collapsed forks. MMS-induced Chk1 phosphorylation and Rad22/Rad52 focus formation occurred normally, whereas cells containing Rhp54/Rad54 foci, which are involved in DNA strand exchange and dissociation of the joint molecules, were increased. Remarkably, G1 phase extension through deletion of an S phase cyclin, Cig2, as well as Cdt1 overexpression restored pre-RC assembly and suppressed Rhp54 accumulation. A cdc18 mutation also caused hypersensitivity to MMS and CPT and accumulation of Rhp54 foci. These data suggest that an abundance of pre-RCs facilitates a late step in the recombinational repair of collapsed forks in the following S phase. Background: Assembly of many prereplicative complexes (pre-RCs) is important for DNA replication and surviving replication stress. Results: A reduction of pre-RCs caused accumulation of Rhp54/Rad54 foci when progression of replication was hindered. Conclusion: An abundance of pre-RCs facilitates recombinational repair under replication stress. Significance: This study provides a link between pre-RCs and DNA recombination in S phase.