Nucleotide cDNA and complete deduced amino acid sequence of a Trypanosoma cruzi ribosomal P protein (P-JL5)

• Published in: Nucleic acids research 1990-06, Vol.18 (11), p.3399-3399
• Main Authors: SCHIJMAN, A. G, DUSETTI, N. J, VAZQUEZ, M. P, LAFON, S, LEVY-YEYATI, P, LEVIN, M. J
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Genes. Genome; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Protozoan Proteins - genetics; Ribosomal Proteins - genetics; Trypanosoma cruzi - genetics
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/18.11.3399

The isolation of a T. cruzi lambda gt11 recombinant cDNA clone, JL5, which reacted predominantly with chronic Chagas heart disease sera has been reported. The nucleotide and deduced amino acid sequence allowed identification as the C-terminal portion of a T. cruzi ribosomal P protein. Radioactive JL5 cDNA probes were used to screen a T. cruzi Tul2 stock epimastigote cDNA library. A recombinant was isolated, P-JL5, that encodes the complete amino acid sequence of the JL5 P protein. The underlined 17 bases at the 5' end represent the 3' region of the mini-exon sequence. The ORF of 107 amino acids begins at position 30. The first 7 N-terminal residues are 100% homologous to the yeast A2 P protein. The last 10 C-terminal residues define the distinctive consensus P protein motif. The serine at position 84 differs from the glycine reported for the JL5 sequence, due to the nucleotide change A(P-JL5) to G(JL5).