Inhibitory Effect of Curcumol on Jak2-STAT Signal Pathway Molecules of Fibroblast-Like Synoviocytes in Patients with Rheumatoid Arthritis

Hyperplasia of synovial membrane in rheumatoid arthritis (RA) is a critical pathological foundation for inducing articular injury. The janus kinase and signal transducer and activator of transcription (Jak-STAT) pathway plays a critical role in synovial membrane proliferation induced by platelet-der...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2012-01, Vol.2012 (2012), p.1-8
Main Authors: Peng, Daizhi, Shi, Ying, Zou, Qinghua, Wang, Zhizhong, Wang, Yong, Fang, Yongfei, Wang, Heng, Chen, Juerong
Format: Article
Language:English
Subjects:
Cell culture
Cell cycle
Cell growth
Cell proliferation
Curcuma
Cytokines
Cytotoxicity
Disease
DNA biosynthesis
Electrophoretic mobility
Ethanol
Fibroblasts
Flow cytometry
Fluorescence
Growth factors
Hyperplasia
Immunology
Janus kinase
Janus kinase 2
Kinases
Molecular chains
Patients
Phosphorylation
Plant extracts
Platelet-derived growth factor
Platelet-derived growth factor BB
Proteins
Rheumatism
Rheumatoid arthritis
Signal transduction
Stat1 protein
Stat3 protein
Synovial membrane
Synoviocytes
Transcription factors
Tumors
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Summary:Hyperplasia of synovial membrane in rheumatoid arthritis (RA) is a critical pathological foundation for inducing articular injury. The janus kinase and signal transducer and activator of transcription (Jak-STAT) pathway plays a critical role in synovial membrane proliferation induced by platelet-derived growth factor (PDGF). To explore the anti-cell proliferation mechanism of curcumol, a pure monomer extracted from Chinese medical plant zedoary rhizome, the changes of Jak2-STAT1/3 signal pathway-related molecules in synoviocytes were observed in vitro. In this study, the fibroblast-like synoviocytes (FLS) in patients with RA were collected and cultured. The following parameters were measured: cell proliferation (WST-1 assay), cell cycles (fluorescence-activated cell sorting, FACS), STAT1 and STAT3 activities (electrophoretic mobility shift assay, EMSA), and the protein expressions of phosphorylated Jak2, STAT1, and STAT3 (Western blot). It was shown that curcumol could inhibit the RA-FLS proliferation and DNA synthesis induced by PDGF-BB in a dose-dependent manner in vitro. The transcription factors activities of STAT1 and STAT3 were obviously elevated after PDGF-BB stimulation (P
ISSN:1741-427X
1741-4288
DOI:10.1155/2012/746426