Transfer RNAs of potato (Soanum tuberosum) mitochondria have different genetic origins

Total transfer RNAs were extracted from highly purified potato mitochondria. From quantitative measurements, the in vivo tRNA concentration in mitochondria was estimated to be in the range of 60 microM. Total potato mitochondrial tRNAs were fractionated by two-dimensional polyacrylamide gel electrop...

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Bibliographic Details
Published in:Nucleic acids research 1990-07, Vol.18 (13), p.3689-3696
Main Authors: MARECHAL-DROUARD, L, GUILLEMAUT, P, COSSET, A, ARBOGAST, M, WEBER, F, WEIL, J.-H, DIETRICH, A
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Chloroplasts - analysis
DNA, Mitochondrial - genetics
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Genes, Plant
Mitochondria - analysis
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Plant Proteins - analysis
RNA, Ribosomal - analysis
RNA, Transfer - analysis
RNA, Transfer - genetics
Solanum tuberosum
Solanum tuberosum - genetics
Translation. Translation factors. Protein processing
Triticum aestivum
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Summary:Total transfer RNAs were extracted from highly purified potato mitochondria. From quantitative measurements, the in vivo tRNA concentration in mitochondria was estimated to be in the range of 60 microM. Total potato mitochondrial tRNAs were fractionated by two-dimensional polyacrylamide gel electrophoresis. Thirty one individual tRNAs, which could read all sense codons, were identified by aminoacylation, sequencing or hybridization to specific oligonucleotides. The tRNA population that we have characterized comprises 15 typically mitochondrial, 5 'chloroplast-like' and 11 nuclear-encoded species. One tRNA(Ala), 2 tRNAs(Arg), 1 tRNA(Ile), 5 tRNAs(Leu) and 2 tRNAs(Thr) were shown to be coded for by nuclear DNA. A second, mitochondrial-encoded, tRNA(Ile) was also found. Five 'chloroplast-like' tRNAs, tRNA(Trp), tRNA(Asn), tRNA(His), tRNA(Ser)(GGA) and tRNA(Met)m, presumably transcribed from promiscuous chloroplast DNA sequences inserted in the mitochondrial genome, were identified, but, in contrast to wheat (1), potato mitochondria do not seem to contain 'chloroplast-like' tRNA(Cys) and tRNA(Phe). The two identified tRNAs(Val), as well as the tRNA(Gly), were found to be coded for by the mitochondrial genome, which again contrasts with the situation in wheat, where the mitochondrial genome apparently contains no tRNA(Val) or tRNA(Gly) gene (2).
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.13.3689