A non-curved chicken lysozyme 5' matrix attachment site is 3' followed by a strongly curved DNA sequence

Matrix attachment regions (MARs) partition the genome into functional and structural loop-domains. Here, we determined the relative matrix affinity of cloned fragments of the chicken lysozyme 5' MAR. We show that this region contains a non-curved high-affinity binding site, which is 3' fol...

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Bibliographic Details
Published in:Nucleic acids research 1990-07, Vol.18 (13), p.3881-3885
Main Authors: VON KRIES, J. P, PHI-VAN, L, DIEKMANN, S, STRÄTLING, W. H
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding Sites
Biological and medical sciences
Chickens
Chromatin. Chromosome
Cloning, Molecular
DNA - genetics
DNA - metabolism
Female
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Muramidase - metabolism
Nuclear Matrix - metabolism
Nucleic Acid Conformation
Restriction Mapping
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Summary:Matrix attachment regions (MARs) partition the genome into functional and structural loop-domains. Here, we determined the relative matrix affinity of cloned fragments of the chicken lysozyme 5' MAR. We show that this region contains a non-curved high-affinity binding site, which is 3' followed by a strongly curved DNA sequence that exhibits weak matrix binding. DNA curvature is not a physical property required for strong matrix binding. Possible biological functions of this sequence arrangement, particularly of the strongly curved DNA, are discussed.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.13.3881