Complete primary structure of lamb preprochymosin deduced from cDNA

A cDNA coding for lamb preprochymosin was isolated from an abomasum cDNA library successively screened with two oligonucleotide probes corresponding to the 5'- and 3'-ends of the coding region for calf prochymosin. Similar to the situation for the calf enzyme, a 16 amino acid residue signa...

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Bibliographic Details
Published in:Nucleic acids research 1990-08, Vol.18 (15), p.4602-4602
Main Authors: PUNGERCAR, J, STRUKELJ, B, GUBENSEK, F, TURK, V, KREGAR, I
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Chymosin - genetics
DNA - genetics
Enzyme Precursors - genetics
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
preprochymosin
Sequence Homology, Nucleic Acid
Sheep
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Summary:A cDNA coding for lamb preprochymosin was isolated from an abomasum cDNA library successively screened with two oligonucleotide probes corresponding to the 5'- and 3'-ends of the coding region for calf prochymosin. Similar to the situation for the calf enzyme, a 16 amino acid residue signal peptide is followed by 42 residues of the proenzyme region. The mature lamb chymosin begins with glycine at position 59. The coding nucleotide and deduced amino acid sequences of lamb preprochymosin show about 95% and 94% similarity to calf preprochymosin, respectively.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.15.4602