Nucleotide and deduced amino acid sequence of an aspartic proteinase inhibitor homologue from potato tubers (Solanum tuberosum L.)

cDNA sequences of any aspartic proteinase inhibitors have not been reported so far. Two protein inhibitors of aspartic proteinases were isolated from potato tubers and sequenced. In order to find related inhibitors, several cDNA clones have been isolated from a potato tuber lambda gt11 cDNA library...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1990-08, Vol.18 (15), p.4605-4605
Main Authors: STRUKELJ, B, PUNGERCAR, J, RITONJA, A, KRIZAJ, I, GUBENSEK, F, KREGAR, I, TURK, V
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Aspartic Acid Endopeptidases
aspartic proteinase inhibitor
Base Sequence
Biological and medical sciences
Endopeptidases - metabolism
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Plant Proteins - genetics
Protease Inhibitors - metabolism
Proteins - genetics
Sequence Homology, Nucleic Acid
Solanum tuberosum
Solanum tuberosum - genetics
tubers
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:cDNA sequences of any aspartic proteinase inhibitors have not been reported so far. Two protein inhibitors of aspartic proteinases were isolated from potato tubers and sequenced. In order to find related inhibitors, several cDNA clones have been isolated from a potato tuber lambda gt11 cDNA library encoding aspartic proteinase inhibitor homologues. The longest full-length clone contains an open reading frame of 660 bp coding for a protein of 220 amino acid residues. The deduced protein sequence shows about 94% and 99% similarity to the novel inhibitor of cathepsin D and potato cathepsin D inhibitor, respectively.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.15.4605