The Effect of Nano-Scale Topography on Keratinocyte Phenotype and Wound Healing Following Burn Injury

Topographic modulation of tissue response is an important consideration in the design and manufacture of a biomaterial. In developing new tissue therapies for skin, all levels of architecture, including the nanoscale need to be considered. Here we show that keratinocyte phenotype is affected by nano...

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Bibliographic Details
Published in:Tissue engineering. Part A 2012-04, Vol.18 (7-8), p.73-714
Main Authors: Parkinson, Leigh G., Rea, Suzanne M., Stevenson, Andrew W., Wood, Fiona M., Fear, Mark W.
Format: Article
Language:English
Subjects:
Aluminum Oxide
Animals
Biomaterials
Burns
Cell Line
Cell Movement - physiology
Cell Proliferation
Cytology
Dressings
Female
Hogs
Humans
Injuries
Keratinocytes
Keratinocytes - cytology
Leukocyte migration
Original
Original Articles
Pores
Skin
Swine
Tissue engineering
Topography
Wound healing
Wound Healing - physiology
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Summary:Topographic modulation of tissue response is an important consideration in the design and manufacture of a biomaterial. In developing new tissue therapies for skin, all levels of architecture, including the nanoscale need to be considered. Here we show that keratinocyte phenotype is affected by nanoscale changes in topography with cell morphology, proliferation, and migration influenced by the pore size in anodic aluminum oxide membranes. A membrane with a pore size of 300 nm, which enhanced cell phenotype in vitro, was used as a dressing to cover a partial thickness burn injury in the pig. Wounds dressed with the membrane showed evidence of advanced healing with significantly less organizing granulation tissue and more mature epidermal layers than control wounds dressed with a standard burns dressing. The results demonstrate the importance of nanoscale topography in modulating keratinocyte phenotype and skin wound healing.
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2011.0307