Rapid cloning by homologous recombination in vivo

Cloning techniques are fundamental to molecular biology. Classically, recombinant plasmid and/or phage vectors are prepared in vitro, the transformation step only serving for amplification purposes. A different approach would exploit the natural intracellular enzymatic machinery to produce recombina...

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Bibliographic Details
Published in:Nucleic acids research 1993-07, Vol.21 (15), p.3601-3602
Main Authors: Bubeck, Peter, Winkler, Monica, Bautsch, Wilfried
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Base Sequence
Biological and medical sciences
Cloning, Molecular - methods
DNA, Recombinant
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Molecular Sequence Data
Nucleic acids
Plasmids
Polymerase Chain Reaction
Transformation, Bacterial
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Summary:Cloning techniques are fundamental to molecular biology. Classically, recombinant plasmid and/or phage vectors are prepared in vitro, the transformation step only serving for amplification purposes. A different approach would exploit the natural intracellular enzymatic machinery to produce recombinant DNA molecules. Such techniques are well-known to mutagenize chromosomal DNA by e.g. transposon mutagenesis but uncommon yet in the production of recombinant plasmid molecules. Here, we describe a novel cloning method which seems to be mediated by homologous recombination.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/21.15.3601