Two polymorphic TaqI sites at the human NGFR locus (17q12→17q22)

The cosmid clone C3 contains human NGFR sequences cloned in the JB8 vector. TaqI (TCGA) identifies two site dimorphisms. Constant bands are observed at 6.5 kb, 3.2 kb, 2.3 kb, 2.0 kb, 1.15 kb and 0.85 kb. Combined heterozygosity is 55%. The NGFR locus has been assigned to 17q12->17q22 with somati...

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Bibliographic Details
Published in:Nucleic acids research 1989-01, Vol.17 (2), p.824-824
Main Authors: WRIGHT, E. C, FAIN, P. R, BARKER, D. F, CHAO, M. V
Format: Article
Language:English
Subjects:
550200 - Biochemistry
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
Chromosome Mapping
CHROMOSOMES
Chromosomes, Human, Pair 17
CLONING
Deoxyribonucleases, Type II Site-Specific
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
Fundamental and applied biological sciences. Psychology
Genes. Genome
GENETIC MAPPING
HETEROCHROMOSOMES
HUMAN POPULATIONS
Humans
HYBRIDIZATION
MAPPING
Molecular and cellular biology
Molecular genetics
Nerve Growth Factors - metabolism
NUCLEIC ACIDS
ORGANIC COMPOUNDS
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
POPULATIONS
Receptors, Cell Surface - genetics
Receptors, Nerve Growth Factor
RECOMBINANT DNA
RFLPS
STRUCTURAL CHEMICAL ANALYSIS
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Description
Summary:The cosmid clone C3 contains human NGFR sequences cloned in the JB8 vector. TaqI (TCGA) identifies two site dimorphisms. Constant bands are observed at 6.5 kb, 3.2 kb, 2.3 kb, 2.0 kb, 1.15 kb and 0.85 kb. Combined heterozygosity is 55%. The NGFR locus has been assigned to 17q12->17q22 with somatic cell hybrid analysis and in situ hybridization. The TaqI site polymorphisms show tight linkage to HOX2 and other 17q markers and loose linkage to NFl. No significant deviation from Hardy-Weinberg equilibrium has been observed for either system. Proper Mendelian segregation has been observed in 45 offspring of parents segregating system A and 31 offspring of parents segregating B alleles.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.2.824