MspI RFLP at CRYB1 locus (17q11.2→17q12)

The probe pbeta8-2 is a 1.7 kb Msp1 fragment cloned into the AccI site of pUC8. The fragment was derived from the clone Lambda18A (1). MspI (CCGG) identifies two alleles with lengths of 2.0 and 1.7 kb respectively. No constant bands are present. CRYB1 has been assigned to 17q11.2->17q12. The loca...

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Bibliographic Details
Published in:Nucleic acids research 1989-01, Vol.17 (2), p.827-827
Main Authors: BARKER, D. F, FAIN, P. R, WRIGHT, E. C, NGUYEN, K, TSUI, L.-C
Format: Article
Language:English
Subjects:
550200 - Biochemistry
BACTERIOPHAGES
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
Chromosome Mapping
CHROMOSOMES
Chromosomes, Human, Pair 17
CLONING
Crystallins - genetics
Deoxyribonuclease HpaII
Deoxyribonucleases, Type II Site-Specific
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
Fundamental and applied biological sciences. Psychology
Genes. Genome
GENETIC MAPPING
HETEROCHROMOSOMES
HUMAN POPULATIONS
Humans
HYBRIDIZATION
MAPPING
MICROORGANISMS
Molecular and cellular biology
Molecular genetics
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
POPULATIONS
RECOMBINANT DNA
RFLPS
STRUCTURAL CHEMICAL ANALYSIS
VIRUSES
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Description
Summary:The probe pbeta8-2 is a 1.7 kb Msp1 fragment cloned into the AccI site of pUC8. The fragment was derived from the clone Lambda18A (1). MspI (CCGG) identifies two alleles with lengths of 2.0 and 1.7 kb respectively. No constant bands are present. CRYB1 has been assigned to 17q11.2->17q12. The localization of pbeta8-2 to this region has been confirmed by genetic linkage studies which show this probe to be a member of a group showing tight linkage to NF1 and each other. No significant deviation from Hardy-Weinberg equilibrium was found. Proper Mendelian segregation was observed in 125 offspring of backcross matings and 68 offspring of intercross matings.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.2.827