Primary structure of the Chlorella vulgaris small subunit ribosomal RNA coding region

The small subunit ribosomal RNA gene of Chlorella vulgaris (type strain 211-11b of the Goettingen collection, FRG) was amplified from bulk genomic DNA by a modification of the polymerase chain reaction technique as previously described. Both orientations of the amplified small subunit rRNA coding re...

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Bibliographic Details
Published in:Nucleic acids research 1989-02, Vol.17 (3), p.1255-1255
Main Authors: HUSS, V. A. R, SOGIN, M. L
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Chlorella - genetics
Chlorella vulgaris
Fundamental and applied biological sciences. Psychology
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
RNA, Ribosomal - genetics
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Summary:The small subunit ribosomal RNA gene of Chlorella vulgaris (type strain 211-11b of the Goettingen collection, FRG) was amplified from bulk genomic DNA by a modification of the polymerase chain reaction technique as previously described. Both orientations of the amplified small subunit rRNA coding region were cloned as BamHI/SalI fragments into M13 phage mp18 and mp19. Complete sequences for both strands were determined by initiating dideoxynucleotide sequencing reactions with oligonucleotide primers complementary to conserved regions in eukaryotic small subunit rRNA coding regions.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.3.1255