The nucleotide sequence of the fanD gene encoding the large outer membrane protein involved in the biosynthesis of K99 fimbriae

The open reading frame of fanD codes for a polypeptide of 783 amino acid residues including an amino-terminal signal sequence. The calculated molecular mass of the mature FanD protein is 84549 dalton. The amino acid sequence of the FanD protein was compared with the amino acid sequence of FaeD, the...

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Bibliographic Details
Published in:Nucleic acids research 1989-02, Vol.17 (3), p.1263-1263
Main Authors: ROOSENDAAL, B, DE GRAAF, F. K
Format: Article
Language:English
Subjects:
Bacterial Outer Membrane Proteins - genetics
Bacterial Outer Membrane Proteins - physiology
Base Sequence
Biological and medical sciences
Escherichia coli
Escherichia coli - genetics
Escherichia coli - physiology
Fimbriae, Bacterial - physiology
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genes. Genome
membrane proteins
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
outer membranes
pili
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Summary:The open reading frame of fanD codes for a polypeptide of 783 amino acid residues including an amino-terminal signal sequence. The calculated molecular mass of the mature FanD protein is 84549 dalton. The amino acid sequence of the FanD protein was compared with the amino acid sequence of FaeD, the large outer membrane protein encoded by the K88 operon. Both proteins show a significant sequence homology (28% of the positions contain an identical, predominantly non-charged, amino acid residue). Furthermore, one alpha -helix transmembrane sequence and several comparable transmembrane beta -sheets were predicted. Both proteins contain a disulfide bridge in their amino- and carboxyterminal sequence.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.3.1263