The nucleotide sequence of a flavodoxin-like gene which precedes two ferredoxin genes in Rhodobacter capsulatus

The fdxN gene coding for the 2(4Fe-4S) ferredoxin I of Rhodobacter capsulatus was cloned, sequenced and found to be located in a nif region on the chromosome. Just in front of fdxN, a gene encoding a (2Fe-2S) ferredoxin has been identified and its product characterized. Further upstream, a 600 bp re...

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Bibliographic Details
Published in:Nucleic acids research 1990-09, Vol.18 (17), p.5284-5284
Main Authors: JOUANNEAU, Y, RICHAUD, P, GRABAU, C
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Ferredoxins - genetics
Flavodoxin - genetics
Flavoproteins - genetics
Fundamental and applied biological sciences. Psychology
genes
Genes, Bacterial
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Molecular Weight
Rhodobacter capsulatus
Rhodopseudomonas - genetics
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Summary:The fdxN gene coding for the 2(4Fe-4S) ferredoxin I of Rhodobacter capsulatus was cloned, sequenced and found to be located in a nif region on the chromosome. Just in front of fdxN, a gene encoding a (2Fe-2S) ferredoxin has been identified and its product characterized. Further upstream, a 600 bp region has been sequenced. An ORF of 480 bp was identified beginning with a CTG, ending only 18 bp before the start codon of the (2Fe-2S) ferredoxin gene. This ORF has two possible CTG start codons, the second being more probable since it is located within the correct distance from a putative ribosome binding site. The deduced polypeptide contains 159 or 160 amino acids and has a predicted molecular weight of 17,100-17,200.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.17.5284