A quick and efficient method for the recovery of plasmid or viral DNA from mammalian cells

The use of shuttle vectors to study DNA replication and repair in mammalian cells has created the need for techniques to recover plasmid DNA from mammalian cells. We describe a procedure that is rapid (< 2 h for 12 samples), simple (requires no phenol extractions), and provides DNA that is of a q...

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Bibliographic Details
Published in:Nucleic acids research 1990-10, Vol.18 (20), p.6150-6150
Main Authors: LUTZE, L. H, WINEGAR, R. A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
DNA - genetics
DNA - isolation & purification
DNA Repair
DNA Replication
DNA, Viral - genetics
DNA, Viral - isolation & purification
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Genetic Techniques
Genetic Vectors
Methods. Procedures. Technologies
Plasmids
Transfection
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Summary:The use of shuttle vectors to study DNA replication and repair in mammalian cells has created the need for techniques to recover plasmid DNA from mammalian cells. We describe a procedure that is rapid (< 2 h for 12 samples), simple (requires no phenol extractions), and provides DNA that is of a quality suitable for use directly in bacterial transformations or sequencing.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.20.6150