In vivo dynamic imaging of intestinal motions using diet-related autofluorescence

Background  Tissue background autofluorescence induced by standard murine diets containing chlorophyll is a significant problem for fluorescence whole‐body imaging. However, as red chlorophyll autofluorescence delineates the gastrointestinal (GI) tract in the abdomen of the mouse, it should be possi...

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Bibliographic Details
Published in:Neurogastroenterology and motility 2012-05, Vol.24 (5), p.494-497
Main Authors: Kwon, S., Davies-Venn, C., Sevick-Muraca, E. M.
Format: Article
Language:English
Subjects:
Abdomen
Animals
autofluorescence
Chlorophyll
Chlorophyll - chemistry
Contractility
Data processing
Diet
Diets
Digestion
Fluorescence
fluorescence imaging
Gastrointestinal Motility - physiology
Gastrointestinal tract
Intestinal motility
intestinal motion
Intestine, Small - anatomy & histology
Intestine, Small - physiology
Intestines - anatomy & histology
Intestines - physiology
Lasers
Light effects
Mice
Peristalsis
Small intestine
Spectroscopy, Near-Infrared
Velocity
Waves
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Summary:Background  Tissue background autofluorescence induced by standard murine diets containing chlorophyll is a significant problem for fluorescence whole‐body imaging. However, as red chlorophyll autofluorescence delineates the gastrointestinal (GI) tract in the abdomen of the mouse, it should be possible to dynamically and non‐invasively image intestinal motions. Herein, we non‐invasively imaged for the first time intestinal motions, such as peristaltic and segmental motions, without an exogenous imaging agent, using red chlorophyll fluorescence. Methods  Mice were illuminated with 660‐nm light from a laser diode and autofluorescence at 710 nm was acquired dynamically for 5 min with 200‐ms exposure time. Fluorescent imaging data were analyzed to generate a three‐dimensional spatiotemporal map to quantitate intestinal motions. Key Results  Peristaltic and segmental motions were observed in vivo in mice. Our quantification showed that the frequency and propagation velocity of peristaltic contractile waves in the small intestine were measured to be 28.6 cycles per min and 1.82 ± 0.56 cm s−1, respectively. Conclusions & Inferences  This simple, but unexplored imaging technique can provide a means to monitor intestinal motility disorders and response to therapeutic agents.
ISSN:1350-1925
1365-2982
DOI:10.1111/j.1365-2982.2012.01886.x