Analysis of three different repeated DNA elements present in the P1 operon of Mycoplasma pneumoniae : size, number and distribution on the genome

Mycoplasma pneumoniae, a bacterium pathogenic for humans, has a relatively small genome size of 840 kbp. Even though, several repeated DNA elements have been identified in the genome of this prokaryote, particularly within the P1 gene which codes for a major adhesin protein of M. pneumoniae. These e...

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Bibliographic Details
Published in:Nucleic acids research 1990-11, Vol.18 (21), p.6311-6317
Main Authors: RULAND, K, WENZEL, R, HERRMANN, R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Southern
Cloning, Molecular - methods
Cosmids
DNA Probes
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genes. Genome
Genomic Library
Molecular and cellular biology
Molecular genetics
Mycoplasma pneumoniae
Mycoplasma pneumoniae - genetics
Operon
Repetitive Sequences, Nucleic Acid
Restriction Mapping
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Summary:Mycoplasma pneumoniae, a bacterium pathogenic for humans, has a relatively small genome size of 840 kbp. Even though, several repeated DNA elements have been identified in the genome of this prokaryote, particularly within the P1 gene which codes for a major adhesin protein of M. pneumoniae. These elements were characterized in detail with respect to size, number and distribution on the genome, represented by an ordered cloned library covering the complete chromosome. Three different repetitive elements were detected in and around the P1 gene designated as RepMP2/3, RepMP4 and RepMP5. The length of these elements varies between 1.1-1.5 kbp (RepMP4), 1.8 kbp (RepMP2/3) and 1.9-2.2 kpb (RepMP5). They occur at least 8 to 10 times on the chromosome. Possible functions are discussed and a uniform nomenclature for these repeats is proposed.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.21.6311