Transcriptional regulation of gene expression in Tetrahymena thermophila

The only well-characterized study of gene expression in Tetrahymena thermophila (1) demonstrates that the temperature dependent expression of the Ser H3 gene is regulated at the level of mRNA stability. A run-on transcription assay was developed to determine if regulation of RNA stability was a majo...

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Bibliographic Details
Published in:Nucleic acids research 1990-11, Vol.18 (22), p.6637-6639
Main Authors: STARGELL, L. A, KARRER, K. M, GOROVSKY, M. A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Molecular and cellular biology
Molecular genetics
RNA, Messenger - metabolism
Tetrahymena - genetics
Tetrahymena - growth & development
Tetrahymena thermophila
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
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Summary:The only well-characterized study of gene expression in Tetrahymena thermophila (1) demonstrates that the temperature dependent expression of the Ser H3 gene is regulated at the level of mRNA stability. A run-on transcription assay was developed to determine if regulation of RNA stability was a major mechanism regulating gene expression in Tetrahymena or if transcriptional regulation dominates. The relative transcriptional activities of 14 Tetrahymena genes were determined in different physiological/developmental states (growing, starved and conjugating) in which many of the genes showed striking differences in RNA abundance. In every case except Ser H3, changes in transcription accompanied changes in RNA abundance. Thus differential transcription, not differential RNA degradation, is the major mechanism regulating RNA abundance in Tetrahymena.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.22.6637