cDNA and deduced amino acid sequence of rat liver prehsp60 (chaperonin-60)

We report the complete primary structure of rat liver mitochondrial prehsp60 deduced from cloned cDNA sequences. A lambda gt10 library prepared from adult rat liver mRNA was screened with oligonucleotides based on a human prehsp60 sequence and hybridizing cDNAs were sequenced. The entire coding regi...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1990-12, Vol.18 (23), p.7162-7162
Main Authors: Peralta, D, Hartman, D J, McIntosh, A M, Hoogenraad, N J, Høj, P B
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Chaperonin 60
Chaperonins
DNA - genetics
Heat-Shock Proteins - genetics
Humans
Mitochondria, Liver - metabolism
Molecular Sequence Data
Proteins - genetics
Rats
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report the complete primary structure of rat liver mitochondrial prehsp60 deduced from cloned cDNA sequences. A lambda gt10 library prepared from adult rat liver mRNA was screened with oligonucleotides based on a human prehsp60 sequence and hybridizing cDNAs were sequenced. The entire coding region spans 1722 bases yielding a 573 amino acid primary translation product (M sub(r) 60,941) including a typical leader sequence of 26 residues. The predicted M sub(r) of the processed monomer is 57,912 in agreement with immunoblots of rat liver mitochondria using anti-hsp60 antibodies. The deduced amino acid sequence differs from that of human and Chinese hamster prehsp60 in 15 and 7 positions, respectively. (DBO)
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.23.7162