The octamer binding factor Oct6 : cDNA cloning and expression in early embryonic cells

We have cloned a cDNA encoding a novel octamer binding factor Oct6 that is expressed in undifferentiated ES cells. Expression of the Oct6 gene is downregulated upon differentiation of these cells by aggregate formation. Furthermore the gene is transiently up regulated during retinoic acid induced di...

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Bibliographic Details
Published in:Nucleic acids research 1990-12, Vol.18 (24), p.7357-7365
Main Authors: MEIJER, D, GRAUS, A, KRAAY, R, LANGEVELD, A, MULDER, M. P, GROSVELD, G
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Blotting, Northern
Cell Differentiation
Cloning, Molecular
DNA - genetics
DNA - metabolism
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Embryonal Carcinoma Stem Cells
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genes, Homeobox
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Molecular Weight
Multigene Family
Neoplastic Stem Cells
Octamer Transcription Factor-6
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription. Transcription factor. Splicing. Rna processing
Tretinoin - pharmacology
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Summary:We have cloned a cDNA encoding a novel octamer binding factor Oct6 that is expressed in undifferentiated ES cells. Expression of the Oct6 gene is downregulated upon differentiation of these cells by aggregate formation. Furthermore the gene is transiently up regulated during retinoic acid induced differentiation of P19 EC cells, reaching maximum levels of expression one day after RA addition. Sequence analysis of the cDNA encoding the Oct6 protein indicated that the Oct6 gene is a member of the POU-HOMEO domain gene family. The gene expresses a 3 kb mRNA encoding a 449 amino acid protein with an apparent molecular weight of 45 kD. The sequence of the Oct6 POU domain is identical to that of the rat SCIP (Tst-1) gene. The Oct6 expression pattern suggests a role for this DNA binding protein in neurogenesis as well as early embryogenesis.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.24.7357