The cDNA sequence of the α-subunit of the Chinese hamster adenylate cyclase-stimulatory G-protein

A cDNA library from a CCL39 cell line (Chinese hamster lung fibroblasts) was screened with a super(32)P-labeled oligonucleotide. The sequence of the probe was defined as a consensus sequence of the phosphate binding loop of the alpha subunit of GTP-binding proteins of rat, bovine and human). Five cl...

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Bibliographic Details
Published in:Nucleic acids research 1990-02, Vol.18 (3), p.662-662
Main Authors: MERCKEN, L, MORAS, V, TOCQUE, B, MAYAUX, J.-F
Format: Article
Language:English
Subjects:
adenylate cyclase
Adenylyl Cyclases - metabolism
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Cattle
Cell Line
Cricetinae
DNA - genetics
Fibroblasts
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
GTP-Binding Proteins - genetics
GTP-Binding Proteins - physiology
guanine nucleotide-binding protein
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Rats
Sequence Homology, Nucleic Acid
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Summary:A cDNA library from a CCL39 cell line (Chinese hamster lung fibroblasts) was screened with a super(32)P-labeled oligonucleotide. The sequence of the probe was defined as a consensus sequence of the phosphate binding loop of the alpha subunit of GTP-binding proteins of rat, bovine and human). Five clones that hybridized also with an alpha sub(s) specific oligonucleotide, were purified and further characterized. They correspond to the alpha sub(1s) form of the adenylate cyclase stimulatory G-protein. The nucleotide sequence corresponding to the coding region of the Chinese hamster is 96% and 93% homologous to the rat and bovine respectively.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.3.662