A complex RNA sequence determines the internal initiation of encephalomyocarditis virus RNA translation

Translation initiation on EMCV RNA occurs via binding of ribosomes to an internal sequence within the 5' noncoding region. To investigate the organization of the internal ribosome entry site (IRES) we have determined the translational efficiencies of a series of deletion mutants within the 5�...

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Bibliographic Details
Published in:Nucleic acids research 1991-02, Vol.19 (3), p.665-671
Main Authors: Evstafieva, A G, Ugarova, T Y, Chernov, B K, Shatsky, I N
Format: Article
Language:English
Subjects:
Binding Sites
Binding, Competitive
Cloning, Molecular
DNA Mutational Analysis
Encephalomyocarditis virus
Encephalomyocarditis virus - genetics
Hydrogen Bonding
Molecular Structure
Protein Biosynthesis
Regulatory Sequences, Nucleic Acid
Restriction Mapping
Ribosomes - metabolism
RNA, Messenger - genetics
RNA, Messenger - ultrastructure
RNA, Viral - genetics
RNA, Viral - ultrastructure
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Summary:Translation initiation on EMCV RNA occurs via binding of ribosomes to an internal sequence within the 5' noncoding region. To investigate the organization of the internal ribosome entry site (IRES) we have determined the translational efficiencies of a series of deletion mutants within the 5' noncoding region of EMCV RNA. Three functional regions have been distinguished: a sequence between nts 315-484 and the upper parts of the double-helical structural domains III (nts 488-647) and IV (nts 701-763). The first one greatly enhances translation, but is not absolutely necessary for internal initiation. The other two regions are indispensable to this process. A sequence within domain IV determines inhibition of in vitro translation of mRNAs with 5'-terminal dependent initiation. It is proposed to interact with a translational factor(s) common to the internal and 5'-terminal dependent initiation.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.3.665